The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
ICC/IF: 1/100 - 1/1000.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
Predominantly expressed in brain. Selectively expressed in neurons.
ab39713 detects a band at 85 kDa in mouse and rat brain tissue lysate. This band corresponds to the size of the HAP1-B protein detected in rat brain tissue lysate by Shi-Hua Li et al. (1998). (PMID: 9798945).