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Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
Recognises both A and B isoforms of Huntingtin Associated Protein 1 (HAP1)
IHC (PFA fixed)
IP more details
Mouse, Rat, Monkey
Recombinant fusion protein of Rat Huntingtin Associated Protein 1.
ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
This antibody clone is manufactured by Abcam.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Light chain type
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
ICC/IF: 1/100 - 1/1000.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
Predominantly expressed in brain. Selectively expressed in neurons.
Contains 1 HAP1 N-terminal domain.
Information by UniProt
HAP 1 antibody
HAP 1A antibody
HAP 2 antibody
Anti-Huntingtin Associated Protein 1 antibody [1B6] images
Western blot - Huntingtin Associated Protein 1 antibody [1B6] (ab39713)
All lanes :
Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml
Lane 1 :
Brain (Rat) Tissue Lysate - normal tissue
Lane 2 :
Brain (Mouse) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size :
Observed band size :
85 kDa (
why is the actual band size different from the predicted?
Additional bands at :
50 kDa (possible non-specific secondary antibody binding).
References for Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)
This product has been referenced in:
et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003).
Read more (PubMed: 12763227) »
et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003).
Read more (PubMed: 12754295) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"