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Product name Anti-Huntingtin Associated Protein 1 antibody [1B6] See all Huntingtin Associated Protein 1 primary antibodies
Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
Specificity Recognises both A and B isoforms of Huntingtin Associated Protein 1 (HAP1)
Tested applications Suitable for:
IHC (PFA fixed), IHC-FrFl, ICC/IF, WB, IP more details
Mouse, Rat, Monkey
Recombinant fusion protein of Rat Huntingtin Associated Protein 1.
ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Purity IgG fraction
Clone number 1B6
Light chain type kappa
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
Application notes ICC/IF: 1/100 - 1/1000.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
Tissue specificity Predominantly expressed in brain. Selectively expressed in neurons.
Sequence similarities Contains 1 HAP1 N-terminal domain.
Information by UniProt
HAP 1 antibody
HAP 1A antibody
HAP 2 antibody
Anti-Huntingtin Associated Protein 1 antibody [1B6] images
Western blot - Huntingtin Associated Protein 1 antibody [1B6] (ab39713)
All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml Lane 1 : Brain (Rat) Tissue Lysate - normal tissue Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 75 kDa Observed band size : 85 kDa ( why is the actual band size different from the predicted?) Additional bands at : 50 kDa (possible non-specific secondary antibody binding).
References for Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)
This product has been referenced in:
et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003).
Read more (PubMed: 12763227) »
et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003).
Read more (PubMed: 12754295) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"