Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)

Overview

  • Product nameAnti-Huntingtin Associated Protein 1 antibody [1B6]
    See all Huntingtin Associated Protein 1 primary antibodies
  • Description
    Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
  • SpecificityRecognises both A and B isoforms of Huntingtin Associated Protein 1 (HAP1)
  • Tested applicationsSuitable for: IHC (PFA fixed), IHC-FrFl, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
  • Immunogen

    Recombinant fusion protein of Rat Huntingtin Associated Protein 1.

  • Positive control
    • ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.

Properties

Applications

Our Abpromise guarantee covers the use of ab39713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
IHC-FrFl
ICC/IF
WB
IP
  • Application notesICC/IF: 1/100 - 1/1000.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    IHC-FrFl: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAssociates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
    • Tissue specificityPredominantly expressed in brain. Selectively expressed in neurons.
    • Sequence similaritiesContains 1 HAP1 N-terminal domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • HAP 1 antibody
      • HAP 1A antibody
      • HAP 2 antibody
      • HAP-1 antibody
      • Hap1 antibody
      • HAP1_HUMAN antibody
      • HAP2 antibody
      • HAPP antibody
      • hHAP1 antibody
      • hHLP1 antibody
      • HIP 5 antibody
      • HIP5 antibody
      • HLP antibody
      • HLP1 antibody
      • Huntingtin associated protein 1A antibody
      • Huntingtin-associated protein 1 antibody
      • huntingtin-associated protein 2 antibody
      • Neuroan 1 antibody
      • Neuroan1 antibody
      • OTTHUMP00000164746 antibody
      • OTTHUMP00000164747 antibody
      • OTTHUMP00000232821 antibody
      • OTTHUMP00000232822 antibody
      see all

    Anti-Huntingtin Associated Protein 1 antibody [1B6] images

    • All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate - normal tissue
      Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 75 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific secondary antibody binding).

    References for Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)

    This product has been referenced in:
    • Torre ER  et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003). Read more (PubMed: 12763227) »
    • Gutekunst CA  et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003). Read more (PubMed: 12754295) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"