Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713)

Overview

  • Product name
    Anti-Huntingtin Associated Protein 1 antibody [1B6]
    See all Huntingtin Associated Protein 1 primary antibodies
  • Description
    Mouse monoclonal [1B6] to Huntingtin Associated Protein 1
  • Specificity
    Recognises both A and B isoforms of Huntingtin Associated Protein 1 (HAP1)
  • Tested applications
    Suitable for: IHC (PFA fixed), IHC-FrFl, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
  • Immunogen

    Recombinant fusion protein of Rat Huntingtin Associated Protein 1.

  • Positive control
    • ab39713 gave a strong signal in Mouse Brain Tissue Lysate. The same band was detected in Rat Brain Tissue Lysate, although at a lower intensity.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab39713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
IHC-FrFl
ICC/IF
WB
IP
  • Application notes
    ICC/IF: 1/100 - 1/1000.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    IHC-FrFl: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: 1/100 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
    • Tissue specificity
      Predominantly expressed in brain. Selectively expressed in neurons.
    • Sequence similarities
      Contains 1 HAP1 N-terminal domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • HAP 1 antibody
      • HAP 1A antibody
      • HAP 2 antibody
      • HAP-1 antibody
      • Hap1 antibody
      • HAP1_HUMAN antibody
      • HAP2 antibody
      • HAPP antibody
      • hHAP1 antibody
      • hHLP1 antibody
      • HIP 5 antibody
      • HIP5 antibody
      • HLP antibody
      • HLP1 antibody
      • Huntingtin associated protein 1A antibody
      • Huntingtin-associated protein 1 antibody
      • huntingtin-associated protein 2 antibody
      • Neuroan 1 antibody
      • Neuroan1 antibody
      • OTTHUMP00000164746 antibody
      • OTTHUMP00000164747 antibody
      • OTTHUMP00000232821 antibody
      • OTTHUMP00000232822 antibody
      see all

    Images

    • All lanes : Anti-Huntingtin Associated Protein 1 antibody [1B6] (ab39713) at 1 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate - normal tissue
      Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 75 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific secondary antibody binding).

    References

    This product has been referenced in:
    • Torre ER  et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 125:27-32 (2003). Read more (PubMed: 12763227) »
    • Gutekunst CA  et al. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. J Histochem Cytochem 51:841-52 (2003). Read more (PubMed: 12754295) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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