Anti-Huntingtin Associated Protein 1 antibody (ab58600)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab58600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 76 kDa). An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunising peptide.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
    • Tissue specificity
      Predominantly expressed in brain. Selectively expressed in neurons.
    • Sequence similarities
      Contains 1 HAP1 N-terminal domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • HAP 1 antibody
      • HAP 1A antibody
      • HAP 2 antibody
      • HAP-1 antibody
      • Hap1 antibody
      • HAP1_HUMAN antibody
      • HAP2 antibody
      • HAPP antibody
      • hHAP1 antibody
      • hHLP1 antibody
      • HIP 5 antibody
      • HIP5 antibody
      • HLP antibody
      • HLP1 antibody
      • Huntingtin associated protein 1A antibody
      • Huntingtin-associated protein 1 antibody
      • huntingtin-associated protein 2 antibody
      • Neuroan 1 antibody
      • Neuroan1 antibody
      • OTTHUMP00000164746 antibody
      • OTTHUMP00000164747 antibody
      • OTTHUMP00000232821 antibody
      • OTTHUMP00000232822 antibody
      see all

    Images

    • Anti-Huntingtin Associated Protein 1 antibody (ab58600) at 0.1 µg/ml + Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer).

      Predicted band size: 76 kDa
      Observed band size: 25,70 kDa (why is the actual band size different from the predicted?)



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    ab58600 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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