The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 76 kDa). An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAssociates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat.
Tissue specificityPredominantly expressed in brain. Selectively expressed in neurons.