Anti-hUPF2/RENT2 antibody (ab157108)

Overview

  • Product name
  • Description
    Rabbit polyclonal to hUPF2/RENT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Opossum, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human hUPF2/RENT2 (NP_056357.1).

  • Positive control
    • 293T, HeLa, Jurkat, NIH 3T3, TCMK and 4T1 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab157108 was affinity purified using an epitope specific to hUPF2/RENT2 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 148 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 3 MIF4G domains.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434D222 antibody
    • hUpf2 antibody
    • KIAA1408 antibody
    • MGC138834 antibody
    • MGC138835 antibody
    • Nonsense mRNA reducing factor 2 antibody
    • Regulator of nonsense transcripts 2 antibody
    • RENT2 antibody
    • RENT2_HUMAN antibody
    • Smg 3 antibody
    • Smg 3 homolog nonsense mediated mRNA decay factor antibody
    • Up-frameshift suppressor 2 homolog antibody
    • UPF2 antibody
    • UPF2 regulator of nonsense transcripts homolog antibody
    • UROC1 antibody
    • Urocanate hydratase antibody
    • Yeast Upf2p homolog antibody
    see all

Images

  • All lanes : Anti-hUPF2/RENT2 antibody (ab157108) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 148 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-hUPF2/RENT2 antibody (ab157108) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : TCMK whole cell lysate
    Lane 3 : 4T1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 148 kDa


    Exposure time: 10 seconds
  • Detection of hUPF2/RENT2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157108 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157108 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab157108 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (rat neonatal cardiomyocyte)
Specification
rat neonatal cardiomyocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jul 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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