Anti-hUPF2/RENT2 antibody (ab28712)



  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab28712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 153 kDa (predicted molecular weight: 147 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.


  • Function
    Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA.
  • Tissue specificity
  • Sequence similarities
    Contains 3 MIF4G domains.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434D222 antibody
    • hUpf2 antibody
    • KIAA1408 antibody
    • MGC138834 antibody
    • MGC138835 antibody
    • Nonsense mRNA reducing factor 2 antibody
    • Regulator of nonsense transcripts 2 antibody
    • RENT2 antibody
    • RENT2_HUMAN antibody
    • Smg 3 antibody
    • Smg 3 homolog nonsense mediated mRNA decay factor antibody
    • Up-frameshift suppressor 2 homolog antibody
    • UPF2 antibody
    • UPF2 regulator of nonsense transcripts homolog antibody
    • UROC1 antibody
    • Urocanate hydratase antibody
    • Yeast Upf2p homolog antibody
    see all


  • All lanes : Anti-hUPF2/RENT2 antibody (ab28712) at 1/250 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human hUPF2/RENT2 peptide (ab32332) at 1 µg/ml

    Lysates/proteins at 15 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 147 kDa
    Observed band size: 153 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab28712 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28712, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC image of ab28712 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28712, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab28712 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Gracias por contactarnos.

Tenemos varios anticuerpos en el catálogo contra las proteínas indicadas, pero lamentablemente ninguno de ellos se han testado en Ustilago maydis.

Para WB e IP, le adjunto los links para cada ...

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