Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)

Overview

  • Product name
    Anti-HuR / ELAVL1 antibody [19F12AE12]
    See all HuR / ELAVL1 primary antibodies
  • Description
    Mouse monoclonal [19F12AE12] to HuR / ELAVL1
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Does not react with: Cow
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • Human liver and heart Homogenates; HepG2, HeLa, SH-SY5Y, Jurkat, Rat H4IIE, Mouse MEF, Mouse 3T3 and Rat H9C2 cell lysates; SH-SY5Y and HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab170193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
    • ELAV like 1 antibody
    • ELAV like RNA binding protein 1 antibody
    • ELAV-like protein 1 antibody
    • ELAV1 antibody
    • ELAV1_HUMAN antibody
    • Elavl1 antibody
    • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
    • Hu Antigen R antibody
    • Hu-antigen R antibody
    • Hua antibody
    • HuR antibody
    • MelG antibody
    see all

Images

  • All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/ml

    Lane 1 : Human Liver Homogenate
    Lane 2 : Human Heart Homogenate
    Lane 3 : HepG2 Cell Lysate
    Lane 4 : HeLa Cell Lysate
    Lane 5 : SH-SY5Y Cell Lysate
    Lane 6 : Jurkat Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-mouse IR800 at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 36 kDa
  • All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/ml

    Lane 1 : H9C2 Rat Cell Lysate
    Lane 2 : H4IIE Rat Cell Lysate
    Lane 3 : MEF Mouse Cell Lysate
    Lane 4 : 3T3 Mouse Cell Lysate
    Lane 5 : SH-SY5Y Cell Lysate
    Lane 6 : HepG2 Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-mouse AP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 36 kDa
  • Immunocytochemistry on HeLa cells.

    Cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15minutes). The cells were then incubated with the anti-HuR antibody (5µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody used was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. A cytoplasmic antibody (ab75834) was used as counter stain (green). 10% goat serum was used as a blocking agent for all blocking steps.

    The target protein localizes mainly to the nucleus.

  • Immunocytochemistry on SH-SY5Y cells (Human neuroblastoma cell line).

    Cells cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were then incubated with the anti-HuR antibody (5µg/mL) for 2 hours at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. A cytoplasmic antibody (ab75834) was used as counter stain (Green). 10% Goat serum was used as a blocking agent for all blocking steps.

    The target protein localizes mainly to the nucleus.  

  • Flow cytometric analysis of methanol-fixed HeLa cells labeling HuR / ELAVL1 with ab170193 at 1µg/mL (blue) or an equal amount of an isotype control antibody (red).

References

ab170193 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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