Overview

  • Product name
    Anti-HuR / ELAVL1 antibody [4C8] - ChIP Grade
    See all HuR / ELAVL1 primary antibodies
  • Description
    Mouse monoclonal [4C8] to HuR / ELAVL1 - ChIP Grade
  • Specificity
    ab136542 was specifically selected because of it's abiity to supershift HuR/mRNA ribonucleoprotein complexes. ab136542 does not react with HuD, HuC, or Hel-N1.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, CLIP, IP, ChIP, WB, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide, corresponding to a region within HuR/ ELAVL1.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab200342 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab136542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
CLIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
EMSA Use at an assay dependent concentration.

Target

  • Function
    Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
    • ELAV like 1 antibody
    • ELAV like RNA binding protein 1 antibody
    • ELAV-like protein 1 antibody
    • ELAV1 antibody
    • ELAV1_HUMAN antibody
    • Elavl1 antibody
    • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
    • Hu Antigen R antibody
    • Hu-antigen R antibody
    • Hua antibody
    • HuR antibody
    • MelG antibody
    see all

Images

  • Overlay histogram showing MCF7 cells stained with ab136542 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab136542, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Electrophoretic Mobility Shift Assay of HuR/mRNA complex (Lane 1). The complex is significanlty supershifted by the addition of 10ng of ab136542 (Lane 2).

References

This product has been referenced in:
  • Mihailovich M  et al. miR-17-92 fine-tunes MYC expression and function to ensure optimal B cell lymphoma growth. Nat Commun 6:8725 (2015). IP ; Mouse . Read more (PubMed: 26555894) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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