Anti-HuR / ELAVL1 antibody [4G8] (ab110081)

Overview

  • Product nameAnti-HuR / ELAVL1 antibody [4G8]
    See all HuR / ELAVL1 primary antibodies
  • Description
    Mouse monoclonal [4G8] to HuR / ELAVL1
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-101 of Human HuR/ ELAVL1 with a proprietary tag (AAH03376).

  • Positive control
    • Human Breast, Kidney and Testis Tissue; HeLa cell lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab110081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
    • ELAV like 1 antibody
    • ELAV like RNA binding protein 1 antibody
    • ELAV-like protein 1 antibody
    • ELAV1 antibody
    • ELAV1_HUMAN antibody
    • Elavl1 antibody
    • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
    • Hu Antigen R antibody
    • Hu-antigen R antibody
    • Hua antibody
    • HuR antibody
    • MelG antibody
    see all

Anti-HuR / ELAVL1 antibody [4G8] images

  • Overlay histogram showing MCF7 cells stained with ab110081 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110081, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-HuR / ELAVL1 antibody [4G8] (ab110081) + HeLa cell lysate

    Predicted band size : 36 kDa
  • ab110081, at 10µg/ml, staining HuR / ELAVL1 in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry.
  • ab110081, at 10µg/ml, staining HuR / ELAVL1 in formalin-fixed, paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab110081, at 10µg/ml, staining HuR / ELAVL1 in formalin-fixed, paraffin-embedded Human Testis tissue by Immunohistochemistry.
  • ab110081, at 10µg/ml, staining HuR / ELAVL1 in HeLa cells by Immunocytochemistry.

References for Anti-HuR / ELAVL1 antibody [4G8] (ab110081)

ab110081 has not yet been referenced specifically in any publications.

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