1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labelling HuR / ELAVL1 with ab135740 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells labelling HuR / ELAVL1 with ab135740 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-HuR / ELAVL1 antibody (ab135740)
All lanes : Anti-HuR / ELAVL1 antibody (ab135740) at 1/100 dilution
Lane 1 : Non-transfected 293 cell lysate Lane 2 : 293 cell lysate transiently transfected with the HuR / ELAVL1 gene.