Overview

  • Product name
    Anti-HuR / ELAVL1 antibody
    See all HuR / ELAVL1 primary antibodies
  • Description
    Rabbit polyclonal to HuR / ELAVL1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human HuR/ ELAVL1 protein

  • Positive control
    • Hela, Jurkat, CEM and Ramos cell line lysates. Human brain tissue. Jurkat cells. 293 cell lysates transiently transfected with the HuR / ELAVL1 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab135740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
    • ELAV like 1 antibody
    • ELAV like RNA binding protein 1 antibody
    • ELAV-like protein 1 antibody
    • ELAV1 antibody
    • ELAV1_HUMAN antibody
    • Elavl1 antibody
    • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
    • Hu Antigen R antibody
    • Hu-antigen R antibody
    • Hua antibody
    • HuR antibody
    • MelG antibody
    see all

Images

  • All lanes : Anti-HuR / ELAVL1 antibody (ab135740) at 1/100 dilution

    Lane 1 : HeLa cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : CEM cell line lysate
    Lane 4 : Ramos cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 36 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labelling HuR / ELAVL1 with ab135740 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow cytometric analysis of Jurkat cells labelling HuR / ELAVL1 with ab135740 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • All lanes : Anti-HuR / ELAVL1 antibody (ab135740) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the HuR / ELAVL1 gene.

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 36 kDa

References

ab135740 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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