Anti-HuR / ELAVL1 antibody (ab196626)
Key features and details
- Rabbit polyclonal to HuR / ELAVL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HuR / ELAVL1 antibody
See all HuR / ELAVL1 primary antibodies -
Description
Rabbit polyclonal to HuR / ELAVL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human HuR/ ELAVL1 aa 1 to the C-terminus.
Database link: Q15717 -
Positive control
- U251, HeLa, Jurkat, K562 and PC3 cell lysates. MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Entrez Gene: 363854 Rat
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
see all -
Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
Images
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Immunofluorscence analysis of MCF7 cells labeling HuR / ELAVL1 with ab196626 at 1/100 dilution (top panel). Blue: DAPI for nuclear staining (bottom panel).
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All lanes : Anti-HuR / ELAVL1 antibody (ab196626) at 1/500 dilution
Lane 1 : U251 cell extract
Lane 2 : HeLa cell extract
Lane 3 : Jurkat cell extract
Lane 4 : K562 cell extract
Lane 5 : PC3 cell extract
Developed using the ECL technique.
Predicted band size: 36 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196626 has not yet been referenced specifically in any publications.