The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
ELAV like 1 antibody
ELAV like RNA binding protein 1 antibody
ELAV-like protein 1 antibody
Embryonic lethal abnormal vision drosophila homolog like 1 antibody
Hu Antigen R antibody
Hu-antigen R antibody
Anti-HuR / ELAVL1 antibody images
Immunoprecipitation - Anti-HuR / ELAVL1 antibody (ab54987)This image is courtesy of an anonymous Abreview
ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.