Overview

  • Product name
    Anti-HuR / ELAVL1 antibody
    See all HuR / ELAVL1 primary antibodies
  • Description
    Mouse monoclonal to HuR / ELAVL1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSNGYEDHMA EDCRGDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGHS LGYGFVNYVT AKDAERAINT LNGLRLQSKT IKVSYARPSS , corresponding to amino acids 1-101 of Human HuR/ ELAVL1

Properties

Applications

Our Abpromise guarantee covers the use of ab54987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the RRM elav family.
      Contains 3 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications
      Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
      • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
      • ELAV like 1 antibody
      • ELAV like RNA binding protein 1 antibody
      • ELAV-like protein 1 antibody
      • ELAV1 antibody
      • ELAV1_HUMAN antibody
      • Elavl1 antibody
      • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
      • Hu Antigen R antibody
      • Hu-antigen R antibody
      • Hua antibody
      • HuR antibody
      • MelG antibody
      see all

    Anti-HuR / ELAVL1 antibody images

    • ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.

      See Abreview



    • Predicted band size : 36 kDa
      HuR / ELAVL1 antibody (ab54987) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-HuR / ELAVL1 antibody (ab54987)

    This product has been referenced in:
    • Chai Y  et al. HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer. Cancer Med 5:1588-98 (2016). WB ; Human . Read more (PubMed: 27075229) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (lymphocytes)
    Specification
    lymphocytes
    Total protein in input
    700 µg
    Username

    Abcam user community

    Verified customer

    Submitted Aug 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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