Overview

  • Product name
    Anti-HuR / ELAVL1 antibody
    See all HuR / ELAVL1 primary antibodies
  • Description
    Mouse monoclonal to HuR / ELAVL1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSNGYEDHMA EDCRGDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGHS LGYGFVNYVT AKDAERAINT LNGLRLQSKT IKVSYARPSS , corresponding to amino acids 1-101 of Human HuR/ ELAVL1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab54987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the RRM elav family.
      Contains 3 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications
      Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
      • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
      • ELAV like 1 antibody
      • ELAV like RNA binding protein 1 antibody
      • ELAV-like protein 1 antibody
      • ELAV1 antibody
      • ELAV1_HUMAN antibody
      • Elavl1 antibody
      • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
      • Hu Antigen R antibody
      • Hu-antigen R antibody
      • Hua antibody
      • HuR antibody
      • MelG antibody
      see all

    Images

    • ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.

      See Abreview



    • Predicted band size : 36 kDa
      HuR / ELAVL1 antibody (ab54987) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References

    This product has been referenced in:
    • Chai Y  et al. HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer. Cancer Med 5:1588-98 (2016). WB ; Human . Read more (PubMed: 27075229) »

    See 1 Publication for this product

    Customer reviews and Q&As

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (lymphocytes)
    Specification
    lymphocytes
    Total protein in input
    700 µg
    Username

    Abcam user community

    Verified customer

    Submitted Aug 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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