Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
ELAV like 1 antibody
ELAV like RNA binding protein 1 antibody
ELAV-like protein 1 antibody
Embryonic lethal abnormal vision drosophila homolog like 1 antibody
Hu Antigen R antibody
Hu-antigen R antibody
Immunoprecipitation - Anti-HuR / ELAVL1 antibody (ab54987)This image is courtesy of an anonymous Abreview
ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.