The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 94 kDa).
Use at 2-5 µg/mg of lysate.
FunctionPotential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Tissue specificityAbundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
Sequence similaritiesBelongs to the SAPAP family.
Developmental stageElevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
Post-translational modificationsUbiquitinated, leading to its degradation. Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
Detection of HURP by Western Blot of Immunprecipitate.
ab70743 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70743 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-HURP antibody (ab70743)
has not yet been referenced specifically in any publications.
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