Overview

  • Product name
  • Description
    Rabbit polyclonal to HURP
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 846) of Human HURP (NP_055565.2).

  • Positive control
    • HeLa and 293T whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human Testis. IF/ICC: A549 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab70744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 94 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
    • Tissue specificity
      Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
    • Sequence similarities
      Belongs to the SAPAP family.
    • Developmental stage
      Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
    • Post-translational
      modifications
      Ubiquitinated, leading to its degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAP-5 antibody
      • DAP5 antibody
      • Discs large homolog 7 antibody
      • Disks large associated protein 5 antibody
      • Disks large associated protein DLG7 antibody
      • Disks large-associated protein 5 antibody
      • Disks large-associated protein DLG7 antibody
      • DLG 1 antibody
      • DLG 7 antibody
      • DLG1 antibody
      • DLG7 antibody
      • DLGAP5 antibody
      • DLGP5_HUMAN antibody
      • Drosophila discs large 1 tumor suppressor like antibody
      • Hepatoma up regulated protein antibody
      • Hepatoma up-regulated protein antibody
      • Hepatoma upregulated protein antibody
      • HURP antibody
      • KIAA0008 antibody
      see all

    Anti-HURP antibody images

    • ICC/IF image of ab70744 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70744, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • IHC image of HURP staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70744, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Detection of HURP by Western Blot of Immunprecipitate. ab70744 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70744 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Chemiluminescence with exposure time of 10 seconds.
    • All lanes : Anti-HURP antibody (ab70744) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 94 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

    References for Anti-HURP antibody (ab70744)

    This product has been referenced in:
    • Möckel MM  et al. Xenopus laevis Kif18A is a highly processive kinesin required for meiotic spindle integrity. Biol Open 6:463-470 (2017). Read more (PubMed: 28228376) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry.

    I am happy to confirm that the lot number 584612 belongs to the following product:

    ab70744 (Anti-HURP antibody)

    http://www.abcam.com/index.html?datasheet=70744 (or use the following: http://www....

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up