Overview

  • Product name
  • Description
    Rabbit polyclonal to HUS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human HUS1.
    Database link: O60921

  • Positive control
    • OVCAR3, K652 and 293T cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab186850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 32 kDa.

Target

  • Function
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the HUS1 family.
  • Cellular localization
    Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Checkpoint protein HUS1 antibody
    • hHUS1 antibody
    • HUS1 (S. pombe) checkpoint homolog antibody
    • Hus1 antibody
    • HUS1 checkpoint homolog (S. pombe) antibody
    • HUS1 Checkpoint Protein antibody
    • HUS1 protein antibody
    • HUS1+ - like protein antibody
    • HUS1_HUMAN antibody
    • Hydroxyurea-sensitive 1, S. pombe, homolog of antibody
    see all

Images

  • Immunohistochemistry of paraffin-embedded human stomach cancer using ab186850 at dilution of 1:200 (x400 lens).

  • Immunohistochemistry of paraffin-embedded human kidney using ab186850 at dilution of 1:200 (x400 lens).

  • Immunofluorescence analysis of U2OS cell using ab186850. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186850. Blue DAPI for nuclear staining.
  • All lanes : Anti-HUS1 antibody (ab186850) at 1/500 dilution

    Lane 1 : OVCAR3 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : 293T cell extract

    Predicted band size: 32 kDa

References

ab186850 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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