Overview

  • Product name
    Anti-HUS1B antibody
  • Description
    Rabbit polyclonal to HUS1B
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: LTHKRRPSLTVAVE conjugated to KLH, corresponding to amino acids 104-117 of Human HUS1B (NP_683762.2).

  • Positive control
    • Human Adrenal tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 31 kDa.
ELISA 1/20000 - 1/100000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    This protein is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the checkpoint protein RAD1 but not with RAD9. Over-expression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to the HUS1.
  • Database links
  • Alternative names
    • Checkpoint protein HUS1B antibody
    • hHUS1B antibody
    • HUS1 (S. pombe) checkpoint homolog b antibody
    • HUS1 Checkpoint Homolog B (S. pombe) antibody
    • MGC126746 antibody
    • MGC126748 antibody
    • RP11 532F6.1 antibody
    see all

Images

  • ab115615, at 5µg/ml, staining HUS1B in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115615 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab115615.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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