Overview

  • Product nameAnti-HVCN1 antibody
    See all HVCN1 primary antibodies
  • Description
    Rabbit polyclonal to HVCN1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids of Human HVCN1 (NP_115745.2).

  • Positive control
    • Human spleen and thymus tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab117520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 32 kDa.Can be blocked with HVCN1 peptide (ab214795).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionMediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.
  • Tissue specificityEnriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen.
  • Sequence similaritiesBelongs to the hydrogen channel family.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.
    The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.
  • Post-translational
    modifications
    Phosphorylation may enhance channel gating.
  • Cellular localizationMembrane. Cell membrane. Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types.
  • Information by UniProt
  • Database links
  • Alternative names
    • HV 1 antibody
    • HV1 antibody
    • HVCN 1 antibody
    • HVCN1 antibody
    • HVCN1_HUMAN antibody
    • Hydrogen voltage gated channel 1 antibody
    • Hydrogen voltage-gated channel 1 antibody
    • Voltage gated hydrogen channel 1 antibody
    • Voltage sensor domain only protein antibody
    • Voltage sensor domain-only protein antibody
    • Voltage-gated hydrogen channel 1 antibody
    • VSOP antibody
    see all

Anti-HVCN1 antibody images

  • ab117520, at 5 µg/ml, staining HVCN1 in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry.
  • ab117520, at 5 µg/ml, staining HVCN1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.

References for Anti-HVCN1 antibody (ab117520)

ab117520 has not yet been referenced specifically in any publications.

Product Wall

I am sorry for any confusion.

This protocol is provided from the lab which supplies ab117520 directly to us. It is the same protocol they use to test all their antibodies in IHC butrest assured it is not arandom protocol from IHC world.
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Thank you for your enquiry. Below please find the IHC protocol requested.

I hope this is helpful. Please contact me again if you have any further questions.

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Tissue Preparation

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"