Overview

  • Product nameAnti-HVCN1 antibody
    See all HVCN1 primary antibodies
  • Description
    Rabbit polyclonal to HVCN1
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from N terminal domain of human HVCN1 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab76847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/200.
WB 1/100 - 1/1000. Predicted molecular weight: 32 kDa.

Target

  • FunctionMediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.
  • Tissue specificityEnriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen.
  • Sequence similaritiesBelongs to the hydrogen channel family.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.
    The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.
  • Post-translational
    modifications
    Phosphorylation may enhance channel gating.
  • Cellular localizationMembrane. Cell membrane. Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types.
  • Information by UniProt
  • Database links
  • Alternative names
    • HV 1 antibody
    • HV1 antibody
    • HVCN 1 antibody
    • HVCN1 antibody
    • HVCN1_HUMAN antibody
    • Hydrogen voltage gated channel 1 antibody
    • Hydrogen voltage-gated channel 1 antibody
    • Voltage gated hydrogen channel 1 antibody
    • Voltage sensor domain only protein antibody
    • Voltage sensor domain-only protein antibody
    • Voltage-gated hydrogen channel 1 antibody
    • VSOP antibody
    see all

References for Anti-HVCN1 antibody (ab76847)

ab76847 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (PLB-985, a subclone of HL-60)
Loading amount 1e+006 cells
Specification PLB-985, a subclone of HL-60
Treatment DMFA (5% v/v) for 5 days
Gel Running Conditions Reduced Denaturing (10%)
Blocking step skimmed milk powder (+ 0.1% normal goat serum) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"