Overview

  • Product nameHVEM (NTFRSF14) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    32.77 pg/ml - 8000 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 85.34 69% - 98%
    Serum 90.17 81% - 97%
    Plasma 90.83 73% - 112%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s HVEM Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HVEM in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human HVEM coated on a 96- well plate. Standards and samples are pipetted into the wells and HVEM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HVEM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HVEM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionReceptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • Tissue specificityWidely expressed, with the highest expression in lung, spleen and thymus.
  • Sequence similaritiesContains 3 TNFR-Cys repeats.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession Q92956 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CD270
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator A
    • HveA
    • TNFRSF14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor-like 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

HVEM (NTFRSF14) Human ELISA Kit images

  • Representative Standard Curve using ab113335

Protocols

References for HVEM (NTFRSF14) Human ELISA Kit (ab113335)

ab113335 has not yet been referenced specifically in any publications.

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