Overview

  • Product nameAnti-HYAL1 antibody
    See all HYAL1 primary antibodies
  • Description
    Rabbit polyclonal to HYAL1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (WNANTQWCLE RHGVDVDVSV FDVVANPGQT FRGPDMTIFY SSQLGTYPYY) of Human HYAL1 (NP_695014)

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
  • Tissue specificityHighly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.
  • Involvement in diseaseDefects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9) [MIM:601492]; also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 56 family.
    Contains 1 EGF-like domain.
  • Cellular localizationSecreted. Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • HYAL 1 antibody
    • Hyal-1 antibody
    • HYAL1 antibody
    • HYAL1_HUMAN antibody
    • Hyaluronidase 1 antibody
    • Hyaluronidase-1 antibody
    • Hyaluronoglucosaminidase 1 antibody
    • Hyaluronoglucosaminidase-1 antibody
    • Hyaluronoglucosaminidase1 antibody
    • LUCA 1 antibody
    • LUCA-1 antibody
    • LUCA1 antibody
    • Lung carcinoma protein 1 antibody
    • MGC45987 antibody
    • NAT6 antibody
    • Plasma hyaluronidase antibody
    • Tumor suppressor LUCA 1 antibody
    see all

Anti-HYAL1 antibody images

  • Anti-HYAL1 antibody (ab85375) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
  • ab85375 staining HYAL1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-HYAL1 antibody (ab85375)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (orbital fibroblast)
Specification orbital fibroblast
Treatment 10 ng/ml IL-1beta
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"