Recombinant Anti-HYAL1 antibody [EPR5542] (ab180143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5542] to HYAL1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HYAL1 antibody [EPR5542]
See all HYAL1 primary antibodies -
Description
Rabbit monoclonal [EPR5542] to HYAL1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HYAL1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q12794 -
Positive control
- HepG2, LnCap and HT-1376 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5542 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. -
Tissue specificity
Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues. -
Involvement in disease
Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9) [MIM:601492]; also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. -
Sequence similarities
Belongs to the glycosyl hydrolase 56 family.
Contains 1 EGF-like domain. -
Cellular localization
Secreted. Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 3373 Human
- Omim: 607071 Human
- SwissProt: Q12794 Human
- Unigene: 75619 Human
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Alternative names
- HYAL 1 antibody
- Hyal-1 antibody
- HYAL1 antibody
see all
Images
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All lanes : Anti-HYAL1 antibody [EPR5542] (ab180143) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : LnCap cell lysate
Lane 3 : HT-1376 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5%NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180143 has not yet been referenced specifically in any publications.