Recombinant
RabMAb

Anti-HYAL1 antibody [EPR5542] (ab180143)

Overview

  • Product name
    Anti-HYAL1 antibody [EPR5542]
    See all HYAL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5542] to HYAL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HYAL1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q12794

  • Positive control
    • HepG2, LnCap and HT-1376 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

Target

  • Function
    May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
  • Tissue specificity
    Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.
  • Involvement in disease
    Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9) [MIM:601492]; also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 56 family.
    Contains 1 EGF-like domain.
  • Cellular localization
    Secreted. Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • HYAL 1 antibody
    • Hyal-1 antibody
    • HYAL1 antibody
    • HYAL1_HUMAN antibody
    • Hyaluronidase 1 antibody
    • Hyaluronidase-1 antibody
    • Hyaluronoglucosaminidase 1 antibody
    • Hyaluronoglucosaminidase-1 antibody
    • Hyaluronoglucosaminidase1 antibody
    • LUCA 1 antibody
    • LUCA-1 antibody
    • LUCA1 antibody
    • Lung carcinoma protein 1 antibody
    • MGC45987 antibody
    • NAT6 antibody
    • Plasma hyaluronidase antibody
    • Tumor suppressor LUCA 1 antibody
    see all

Images

  • All lanes : Anti-HYAL1 antibody [EPR5542] (ab180143) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : LnCap cell lysate
    Lane 3 : HT-1376 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)



    Blocking buffer and concentration: 5%NFDM/TBST

References

ab180143 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180143.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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