The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ab66653 detects a band at 50 kDa. This band corresponds in size to the mature cleaved form of HYAL2. Abcam recommends using BSA as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
FunctionHydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
Tissue specificityWidely expressed. No expression detected in adult brain.
Sequence similaritiesBelongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab66653 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab66653 detects a band at 50 kDa. This band corresponds in size to the mature cleaved form of HYAL2.
Western blot - HYAL2 antibody (ab66653)
All lanes : Anti-HYAL2 antibody (ab66653) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Rat Thymus Tissue Lysate Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711) Lane 5 : Raji whole cell lysate (ab30124) Lane 6 : SW480 whole cell lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution