Overview

  • Product nameAnti-HYAL3 antibody
    See all HYAL3 primary antibodies
  • Description
    Mouse polyclonal to HYAL3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length HYAL3 protein (Human)

  • Positive control
    • Hela cells. HYAL3 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab67248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

    NOTE: This product requires purification with Protein A prior to use in ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityBone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells.
    • Sequence similaritiesBelongs to the glycosyl hydrolase 56 family.
      Contains 1 EGF-like domain.
    • Cellular localizationSecreted. Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hyal-3 antibody
      • HYAL3 antibody
      • HYAL3_HUMAN antibody
      • Hyaluronidase 3 antibody
      • Hyaluronidase-3 antibody
      • Hyaluronoglucosaminidase 3 antibody
      • Hyaluronoglucosaminidase-3 antibody
      • LUCA-3 antibody
      • LUCA14 antibody
      • LUCA3 antibody
      • Lung carcinoma protein 3 antibody
      • Minna14 antibody
      see all

    Anti-HYAL3 antibody images

    • All lanes : Anti-HYAL3 antibody (ab67248) at 1/500 dilution

      Lane 1 : HYAL3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
    • Immunofluorescent staining of HYAL3 in HeLa cells using purified ab67248 at 10µg/ml.

    References for Anti-HYAL3 antibody (ab67248)

    ab67248 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (fibroblast)
    Specification fibroblast
    Treatment 10ng/ml IL-1beta 24hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"