Overview

  • Product name
    Anti-Hyaluronic acid antibody
  • Description
    Sheep polyclonal to Hyaluronic acid
  • Specificity
    ab53842 is specific for Hyaluronic acid, a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricating and protective agent.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Dog, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Hyaluronic acid from human umbilical cord

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

See Abreview.

IHC-Fr Use at an assay dependent concentration.

See Abreview.

Target

  • Relevance
    Hyaluronic acid is a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricanting and protective agent.

Images

  • ab53842 staining Hyaluronic acid in chicken cockscomb tissue sections by Immunohistochemistry (formaldehyde fixed sections). Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed using Pronase. Samples were then blocked with 3% H2O2 for 5 minutes at 20°C followed by incubation with the primary antibody at a 1/300 dilution for 1 hour at 20°C. ab6747 Rabbit polyclonal to Sheep IgG - H&L (HRP) was used as secondary antibody, undiluted.

    See Abreview

  • Immunohistochemical analysis of acetone-fixed 10 µm cryostat cut human eplileptic substantia alba tissue, labelling hyaluronic acid with ab53842, incubated at a dilution of 1/250 for 1 hour at 37°C in TNB diluent. Blocking was using a TNB blocking buffer for 30 minutes at 25°C. The secondary used was a polyclonal Alexa Fluor® 488 conjugate used at 1/250.

    See Abreview

References

This product has been referenced in:
  • Polydorou C  et al. Pirfenidone normalizes the tumor microenvironment to improve chemotherapy. Oncotarget 8:24506-24517 (2017). IHC-Fr . Read more (PubMed: 28445938) »
  • Gebauer F  et al. Is hyaluronan deposition in the stroma of pancreatic ductal adenocarcinoma of prognostic significance? PLoS One 12:e0178703 (2017). IHC-P ; Human . Read more (PubMed: 28582436) »

See all 14 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I am not sure what you mean by "revealed system." The secondary antibody used to generate the image on our datasheet was Rabbit polyclonal to Sheep IgG - H&L (HRP) such as ab6747, and it was used with D...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (CNS tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Permeabilization
Yes - Triton X
Specification
CNS tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Martin Broadstock

Verified customer

Submitted Feb 02 2016

We have not tested this antibody against hyaluronic acid which has been degraded by streptococcal hyaluronidase. The immunogen used was highly polymerized human hyaluronic acid as potassium salt isolated from human umbilical cord containing <2% prot...

Read More

Thank you for your updates.
I am very pleased to hear that finally after some optimization steps this antibody works in IHC as it is expected. Thank you for sharing your feedback with us.
If you need any further assistance in the future, plea...

Read More

Thank you for your response.
Normally we do not send a free vial if our customer has run out of the antibody. However, this is a special case and I understand your concern so take your request as an exemption.
This is to let you know that I h...

Read More

Thank you for your enquiry regarding ab53842 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applie...

Read More

Thank you for your patience.

The immunogen used for this antibody was product H1504 from Sigma Aldrich. The antibody binds simply because hyaluronic acid is antigenic; it is the same principle for how antibodies naturally bind bacteria (ie the...

Read More

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period...

Read More

Thank you for contacting us yesterday and your interest in our products.

As discussed over the phone, the anti-Hyaluronic acid antibody (ab53842) has not as yet been used in ICC as you are hoping to do. This does not mean that it would not wo...

Read More

Thank you for your email.

It is possible to raise antibodies to hyaluronic acid in the absence of a carrier protein. This is because hyaluronic acid is a high molecular weight polysaccharide. Such polymers typically have sufficient antigenicit...

Read More

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up