Overview

  • Product name
    Anti-Hyaluronidase PH20 antibody [1A4]
    See all Hyaluronidase PH20 primary antibodies
  • Description
    Mouse monoclonal [1A4] to Hyaluronidase PH20
  • Tested applications
    Suitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Sheep
    Does not react with: Rabbit, Cow, Pig
  • Immunogen

    Ram sperm hyaluronidase monomer

Properties

Applications

Our Abpromise guarantee covers the use of ab50694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.

Target

  • Function
    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • Tissue specificity
    Testis.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 56 family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Alternative names
    • epididymis secretory sperm binding protein Li 96n antibody
    • HEL-S-96n antibody
    • HYA1 antibody
    • HYAL PH-20 antibody
    • HYAL PH20 antibody
    • Hyal-PH20 antibody
    • HYAL1 antibody
    • HYAL3 antibody
    • HYAL5 antibody
    • HYALP_HUMAN antibody
    • Hyaluronidase PH-20 antibody
    • Hyaluronoglucosaminidase PH-20 antibody
    • MGC26532 antibody
    • PH-20 Hyaluronidase antibody
    • PH20 antibody
    • PH20 Hyaluronidase antibody
    • SPAG15 antibody
    • SPAM-1 antibody
    • Spam1 antibody
    • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) antibody
    • Sperm adhesion molecule 1 antibody
    • Sperm surface protein PH-20 antibody
    • Sperm surface protein PH20 antibody
    • zona pellucida binding antibody
    see all

References

This product has been referenced in:
  • Harrison RA Hyaluronidase in ram semen. Quantitative determination, and isolation of multiple forms. Biochem J 252:865-74 (1988). Read more (PubMed: 3421926) »
  • Harrison RA & Gaunt SJ Multiple forms of ram and bull sperm hyaluronidase revealed by using monoclonal antibodies. J Reprod Fertil 82:777-85 (1988). Read more (PubMed: 3361511) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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