Hydrogen Peroxide Blocking Reagent (ab64218)

Overview

  • Product name
    Hydrogen Peroxide Blocking Reagent
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • General notes
    The concentration of this product is 0.3%.

    Immunohistochemistry
    Incubate tissue section for 5-10 minutes at room temperature prior to application of the primary antibody. After incubation rinse twice in buffer.

Properties

Applications

Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Images

  • Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.

     

References

This product has been referenced in:
  • Selby MJ  et al. Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology. PLoS One 11:e0161779 (2016). Read more (PubMed: 27610613) »

See 1 Publication for this product

Customer reviews and Q&As

IHC-P with Ki67 antibody using H2O2 blocking reagant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.
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Abcam user community

Verified customer

Submitted Aug 16 2016

Hydrogen peroxidase Blocking Solution

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.
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Dr. Francesco Elia Marino

Verified customer

Submitted Jul 23 2014


ab64218 and ab94666 are exactly the same products but ab64218 is sold as 125 ml and ab94666 is sold as 60 ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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