Anti-Hydrogen Potassium ATPase Beta antibody [EPR8819(2)] - C-terminal (ab186749)
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- Protocols
Overview
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Product nameAnti-Hydrogen Potassium ATPase Beta antibody [EPR8819(2)] - C-terminal
See all Hydrogen Potassium ATPase Beta primary antibodies -
DescriptionRabbit monoclonal [EPR8819(2)] to Hydrogen Potassium ATPase Beta - C-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P51164 -
Positive control
- Human Hydrogen Potassium ATPase Beta recombinant protein; Human stomach and HepG2 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR8819(2)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab186749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 33 kDa). |
Target
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FunctionRequired for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity.
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Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
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DomainThe C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 496 Human
- Omim: 137217 Human
- SwissProt: P51164 Human
- Unigene: 434202 Human
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Alternative names
- Gastric H(+)/K(+) ATPase subunit beta antibody
- ATP4B antibody
- ATP4B_HUMAN antibody
see all
Images
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Western blot - (ab186749)Anti-Hydrogen Potassium ATPase Beta antibody [EPR8819(2)] - C-terminal (ab186749) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaBlocking buffer: 5% NFDM/TBST
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Western blot - (ab186749)Anti-Hydrogen Potassium ATPase Beta antibody [EPR8819(2)] - C-terminal (ab186749) at 1/5000 dilution + Human stomach lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDaBlocking buffer: 5% NFDM/TBST
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Western blot - (ab186749)Anti-Hydrogen Potassium ATPase Beta antibody [EPR8819(2)] - C-terminal (ab186749) at 1/1000 dilution + Human Hydrogen Potassium ATPase Beta recombinant full length protein with proprietary tag at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaBlocking buffer: 5% NFDM/TBST
Protocols
References
ab186749 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"