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DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Binding Solution||1 x 5ml||1 x 10ml|
|Capture Antibody, 1000 µg/mL||1 x 4µl||1 x 8µl|
|Detection Antibody, 400 µg/mL||1 x 8µl||1 x 16µl|
|Enhancer Solution||1 x 8µl||1 x 16µl|
|Fluoro Developer||1 x 8µl||1 x 16µl|
|Fluoro Dilutor||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 8µl||1 x 16µl|
|Negative Control I, 20 µg/mL||1 x 10µl||1 x 20µl|
|Negative Control II, 20 µg/mL||1 x 10µl||1 x 20µl|
|Positive Control, 20 µg/mL||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab117131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117131 has not yet been referenced specifically in any publications.