Overview

  • Product name
    Anti-Hydroxyproline antibody
  • Description
    Rabbit polyclonal to Hydroxyproline
  • Specificity
    This antibody targets conjugated Trans-Hydroxyproline, it does not recognize free Trans-Hydroxyproline.
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Immunogen

    Synthetic peptide corresponding to Hydroxyproline conjugated to Bovine Serum Albumin (BSA).
    (Peptide available as ab93631)

Properties

Applications

Our Abpromise guarantee covers the use of ab37067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance
    Hydroxyproline, a non-essential amino acid derived from proline, with no known therapeutic use. Hydroxproline is used as a major component of structural protiens such as collagen, connective tissues, plant cell walls, tendons and ligaments and provides skin elasticity. Vitiman C is required for the conversion process from proline to hydroxyproline, a deficincy in vitiman C can lead to defects in collagen synthesis, thus, resulting in easy bruising, internal bleeding, breakdown of connective tissue of the ligaments and tendons, and increased risk to blood vessel damage. An unusual feature of this amino acid is that, it is not incorporated into collagen during biosynthesis at the ribosomal level, but is formed from proline by a posttranslational modification by an enzymatic hydroxylation reaction.

Images

  • Anti-Hydroxyproline antibody (ab37067) at 1/500 dilution + lysate prepared from HEK293T cells at 1 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    WT flag tagged HIF1a was transfected into HEK293T cells. After 48 hours cells were treated with MG132 or DMSO for 6 hours. Cell lysates were subjected to Immunoprecipitation. Samples were separated in SDS-PAGE for Western blotting.

    Negative control: Flag tagged HIF1a mutant (Proline mutants).

    See Abreview

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (renal cancer cell lines)
Total protein in input
20 µg
Treatment
Ctrl or Prolyl hydroxylase inhibitor DMOG treated
Immuno-precipitation step
Protein G
Specification
renal cancer cell lines
Username

Abcam user community

Verified customer

Submitted Jun 13 2016

Thank you for your inquiry.

I can confirm that ab37067 will detect conjugated Trans-Hydroxyproline.

It will not matter to what Trans-Hydroxyproline is conjugated and therefore we expect ab37067 to also bind collagen chains if they h...

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Thank you for contacting us. The dilution must be optimized by the end user but we would recommend 1/100 as a starting point for IHC.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice o...

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Thank you for you response and for sending addition details regarding your protocol. As always, this product is covered by our Abpromise guarantee we will replace it under our Abpromise guarantee, as you are using it according to specifications list...

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I am sorry about the issues you have been having. Thank you for bringing this to our attention.
Thank you for providing information about your protocol. As company policy, in order to send a replacement we do need to know protocol information. Thi...

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Thank you for providing this information. Having reviewed all the details provided, I believe this product should have given satisfactory results but clearly hasn't. It appears that you may have received a faulty vial. This will be investigated. ...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Having rev...

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Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HEK293T)
Loading amount
1 µg
Specification
HEK293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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