Anti-HSD17B4 antibody (ab97971)
Key features and details
- Rabbit polyclonal to HSD17B4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HSD17B4 antibody
See all HSD17B4 primary antibodies -
Description
Rabbit polyclonal to HSD17B4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Pig, Zebrafish -
Immunogen
Recombinant fragment corresponding to Human HSD17B4 aa 18-231.
Database link: P51659 -
Positive control
- WB: HeLa, Mouse brain whole cell lysate IHC-P: Human ovarian cancer. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. -
Tissue specificity
Present in many tissues with highest concentrations in liver, heart, prostate and testis. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
D-bifunctional protein deficiency
Perrault syndrome 1 -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 MaoC-like domain.
Contains 1 SCP2 domain. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 3295 Human
- Entrez Gene: 15488 Mouse
- Entrez Gene: 79244 Rat
- Omim: 601860 Human
- SwissProt: P51659 Human
- SwissProt: P51660 Mouse
- SwissProt: P97852 Rat
- Unigene: 406861 Human
see all -
Alternative names
- 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
- 17 beta HSD 4 antibody
- 17 beta HSD IV antibody
see all
Images
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Paraffin embedded human ovarian cancer tissue stained for HSD17B4 using ab97971 at 1/500 dilution in immunohistochemical analysis.
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HeLa cells stained for HSD17B4 (green) using ab97971 at 1/500 dilution in ICC/IF.
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Anti-HSD17B4 antibody (ab97971) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Anti-HSD17B4 antibody (ab97971) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 80 kDa7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97971 has been referenced in 2 publications.
- Murdamoothoo D et al. Tenascin-C immobilizes infiltrating T lymphocytes through CXCL12 promoting breast cancer progression. EMBO Mol Med 13:e13270 (2021). PubMed: 33988305
- Shi X & Fu L Piceatannol inhibits oxidative stress through modification of Nrf2-signaling pathway in testes and attenuates spermatogenesis and steroidogenesis in rats exposed to cadmium during adulthood. Drug Des Devel Ther 13:2811-2824 (2019). PubMed: 31496657