Anti-Hydroxysteroid (17-beta) Dehydrogenase 4 antibody (ab97975)

Overview

  • Product name
    Anti-Hydroxysteroid (17-beta) Dehydrogenase 4 antibody
    See all Hydroxysteroid (17-beta) Dehydrogenase 4 primary antibodies
  • Description
    Rabbit polyclonal to Hydroxysteroid (17-beta) Dehydrogenase 4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 355-615 of Human hydroxysteroid (17-beta) dehydrogenase 4 (NP_000405).

  • Positive control
    • HepG2 whole cell lysate; DLD-1 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
  • Tissue specificity
    Present in many tissues with highest concentrations in liver, heart, prostate and testis.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease
    D-bifunctional protein deficiency
    Perrault syndrome 1
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 1 MaoC-like domain.
    Contains 1 SCP2 domain.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
    • 17 beta HSD 4 antibody
    • 17 beta HSD IV antibody
    • 17 beta hydroxysteroid dehydrogenase 4 antibody
    • 17-beta-HSD 4 antibody
    • 17-beta-hydroxysteroid dehydrogenase 4 antibody
    • 17beta estradiol dehydrogenase type IV antibody
    • 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody
    • 3-alpha antibody
    • 7-alpha antibody
    • Beta hydroxyacyl dehydrogenase antibody
    • Beta keto reductase antibody
    • D 3 hydroxyacyl CoA dehydratase antibody
    • D bifunctional protein antibody
    • D bifunctional protein peroxisomal antibody
    • D-3-hydroxyacyl CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein antibody
    • D-bifunctional protein antibody
    • D-bifunctional protein, peroxisomal antibody
    • DBP antibody
    • DBP, peroxisomal antibody
    • DHB4_HUMAN antibody
    • EDH17B4 antibody
    • Enoyl-CoA hydratase 2 antibody
    • Hsd17b4 antibody
    • MFE 2 antibody
    • MFE-2 antibody
    • MPF-2 antibody
    • Multifunctional protein 2 antibody
    • Peroxisomal multifunctional enzyme type 2 antibody
    • Peroxisomal multifunctional protein 2 antibody
    • PRLTS1 antibody
    • SDR8C1 antibody
    • Short chain dehydrogenase/reductase family 8C member 1 antibody
    see all

Images

  • Anti-Hydroxysteroid (17-beta) Dehydrogenase 4 antibody (ab97975) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 80 kDa
    7.5% SDS PAGE
  • Immunohistochemical analysis of hydroxysteroid (17-beta) dehydrogenase 4 in paraffin-embedded DLD-1 xenograft, using ab97975 at 1/500 dilution.
  • Anti-Hydroxysteroid (17-beta) Dehydrogenase 4 antibody (ab97975) at 1/3000 dilution + Rat heart lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size : 80 kDa
  • ab97975 staining hydroxysteroid (17-beta) dehydrogenase 4 in rat heart tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution. 

  • ab97975 staining hydroxysteroid (17-beta) dehydrogenase 4 in rat testis tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution. 

  • ab97975 staining hydroxysteroid (17-beta) dehydrogenase 4 in methanol- fixed Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence) at a 1:500 dilution.

References

ab97975 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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