Overview

  • Product name
  • Description
    Rabbit polyclonal to HYLS1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    MEELLPDGQI WANMDPEERM LAAATAFTHI CAGQGEGDVR REAQSIQYDP YSKASVAPGK RPALPVQLQY PHVESNVPSE TVSEASQRLR KPVMK, corresponding to amino acids 1-95 of Human HYLS1 (Q96M11).

  • Positive control
    • Human cerebral cortex tissue. U-2 OS cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Involvement in disease
    Defects in HYLS1 are the cause of hydrolethalus syndrome type 1 (HLS1) [MIM:236680]. HLS1 is a lethal malformation syndrome leading to stillbirth or death shortly after birth. It is characterized by hydrocephaly with absent upper midline structures of the brain, micrognathia and polydactyly.
  • Sequence similarities
    Belongs to the HYLS1 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • HLS antibody
    • Hydrolethalus syndrome protein 1 antibody
    • HYLS1 antibody
    • HYLS1_HUMAN antibody

Images

  • ab122702, at 1/500 dilution, staining HYLS1 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry
  • Immunofluorescent staining of Human cell line U-2 OS using ab122702 at 4µg/ml. Antibody staining is shown in green.

References

ab122702 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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