Overview

  • Product nameAnti-HYPE antibody
    See all HYPE primary antibodies
  • Description
    Rabbit polyclonal to HYPE
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 409-458 (GDVRPFIRFI AKCTETTLDT LLFATTEYSV ALPEAQPNHS GFKETLPVKP) of Human HYPE (NP_009007).

  • Positive control
    • 293T cells transfected with HYPE cell lysate. Human muscle.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionAdenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the fic family.
    Contains 1 fido domain.
    Contains 2 TPR repeats.
  • DomainThe fido domain mediates the adenylyltransferase activity.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine monophosphate-protein transferase FICD antibody
    • AMPylator FICD antibody
    • FIC domain containing antibody
    • FIC domain containing protein antibody
    • FIC domain-containing protein antibody
    • Fic S phase protein cell division homolog antibody
    • ficd antibody
    • FICD_HUMAN antibody
    • HIP-13 antibody
    • HIP13 antibody
    • Huntingtin interacting protein 13 antibody
    • Huntingtin interacting protein E antibody
    • Huntingtin interactor protein E antibody
    • Huntingtin yeast partner E antibody
    • Huntingtin-interacting protein 13 antibody
    • Huntingtin-interacting protein E antibody
    • MGC5623 antibody
    • UNQ3041 antibody
    see all

Anti-HYPE antibody images

  • Anti-HYPE antibody (ab104763) at 0.25 µg/ml + transfected 293T cell lysate at 10 µg

    Predicted band size : 52 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling HYPE with ab104763 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

References for Anti-HYPE antibody (ab104763)

ab104763 has not yet been referenced specifically in any publications.

Product Wall

Thanks for the additional details.

What speices of HYPE is your construct based on?

Thanks for your enquiry.

Just to confirm, ab104782 has been raised against a synthetic peptide immunogen "corresponding to a region within the C terminal amino acids 351-400". This peptide is a 15-20 amino acid peptide found withina...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"