Overview

  • Product nameAnti-HYPE antibody
    See all HYPE primary antibodies
  • Description
    Rabbit polyclonal to HYPE
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 351-400 (FAALAHYKLV YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSDYY) of Human HYPE (NP_009007).

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab104782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAdenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the fic family.
    Contains 1 fido domain.
    Contains 2 TPR repeats.
  • DomainThe fido domain mediates the adenylyltransferase activity.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine monophosphate-protein transferase FICD antibody
    • AMPylator FICD antibody
    • FIC domain containing antibody
    • FIC domain containing protein antibody
    • FIC domain-containing protein antibody
    • Fic S phase protein cell division homolog antibody
    • ficd antibody
    • FICD_HUMAN antibody
    • HIP-13 antibody
    • HIP13 antibody
    • Huntingtin interacting protein 13 antibody
    • Huntingtin interacting protein E antibody
    • Huntingtin interactor protein E antibody
    • Huntingtin yeast partner E antibody
    • Huntingtin-interacting protein 13 antibody
    • Huntingtin-interacting protein E antibody
    • MGC5623 antibody
    • UNQ3041 antibody
    see all

Anti-HYPE antibody images



  • Predicted band size : 52 kDa

References for Anti-HYPE antibody (ab104782)

ab104782 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab104763.

This item is currently out of stock but is expec...

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Thanks for the follow up information.

It is quite interesting that the antibody binds to HYPE but does not appear to bind the region in which the peptide immunogen sequence is located. Its not clear how this couldhappen.

If you woul...

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The 14aa immunizing peptide used to raise this antibody does fall within the 351-400 range of this human protein based on this sequence (http://www.uniprot.org/uniprot/Q9BVA6).

Has the aa187-437 mutantconstruct been sequenced to ensure it ex...

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Thanks for the additional details.

What speices of HYPE is your construct based on?

Thanks for your enquiry.

Just to confirm, ab104782 has been raised against a synthetic peptide immunogen "corresponding to a region within the C terminal amino acids 351-400". This peptide is a 15-20 amino acid peptide found withina...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"