Overview

  • Product name
  • Description
    Mouse polyclonal to HYPE
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HYPE (NP_009007, 1 a.a. ~ 458 a.a) full-length human protein.

  • Positive control
    • Transfected 293T cell lysate, human placenta tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the fic family.
    Contains 1 fido domain.
    Contains 2 TPR repeats.
  • Domain
    The fido domain mediates the adenylyltransferase activity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine monophosphate-protein transferase FICD antibody
    • AMPylator FICD antibody
    • FIC domain containing antibody
    • FIC domain containing protein antibody
    • FIC domain-containing protein antibody
    • Fic S phase protein cell division homolog antibody
    • ficd antibody
    • FICD_HUMAN antibody
    • HIP-13 antibody
    • HIP13 antibody
    • Huntingtin interacting protein 13 antibody
    • Huntingtin interacting protein E antibody
    • Huntingtin interactor protein E antibody
    • Huntingtin yeast partner E antibody
    • Huntingtin-interacting protein 13 antibody
    • Huntingtin-interacting protein E antibody
    • MGC5623 antibody
    • UNQ3041 antibody
    see all

Images

  • Anti-HYPE antibody (ab67163) at 1/500 dilution + human placenta tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • All lanes : Anti-HYPE antibody (ab67163) at 1/500 dilution

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : non Transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References

ab67163 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab67163.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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