Overview

  • Product nameI-TAC (CXCL11) Mouse ELISA KitSee all CXCL11 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    5.487 pg/ml - 4000 pg/ml
  • Recovery

    80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 74.46 67% - 86%
    Serum 91.12 80% - 101%
    Plasma 75.6 68% - 88%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionChemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • Tissue specificityHigh levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession O14625 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • b R1
    • Beta R1
    • Beta-R1
    • betaR1
    • C X C motif chemokine 11
    • C-X-C motif chemokine 11
    • Chemokine (C-X-C motif) ligand 11
    • Chemokine C-X-C motif ligand 11
    • CXCL11
    • CXL11_HUMAN
    • H174
    • I TAC
    • I-TAC
    • Interferon gamma inducible protein 9
    • Interferon gamma-inducible protein 9
    • Interferon inducible T cell a chemoattractant I TAC
    • Interferon inducible T cell alpha chemoattractant
    • Interferon-inducible T-cell alpha chemoattractant
    • IP 9
    • IP-9
    • IP9
    • ITAC
    • MGC102770
    • SCYB11
    • SCYB9B
    • Small inducible cytokine B11
    • Small inducible cytokine subfamily B (Cys X Cys) member 11
    • Small inducible cytokine subfamily B (Cys X Cys) member 9B
    • Small inducible cytokine subfamily B, member 11
    • Small inducible cytokine subfamily B, member 9B
    • Small-inducible cytokine B11
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

I-TAC (CXCL11) Mouse ELISA Kit images

  • Representative standard curve using ab100716

  • Representative standard curve using ab100716

Protocols

References for I-TAC (CXCL11) Mouse ELISA Kit (ab100716)

ab100716 has not yet been referenced specifically in any publications.

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