Anti-iASPP antibody [LXO49.3] (ab49805)

Overview

• Product nameAnti-iASPP antibody [LXO49.3]See all iASPP primary antibodies ...
• Description
  Mouse monoclonal [LXO49.3] to iASPP
• Tested applicationsWB, ICC, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 459-639 of Human iASPP

• Positive controlTotal cell extract of human osteogenic sarcoma (U-2-OS)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 0.01M PBS, pH 7.4
• Concentration information loading...
• Purification notesPurified immunoglobulin
• Clonality Monoclonal
• Clone numberLXO49.3
• MyelomaSp2/0
• IsotypeIgG1
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • p53 pathway

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • p53

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • p53

  • Cell Biology
  • Apoptosis
  • Intracellular
  • p53 Pathway

Applications

Target

• RelevanceASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 10848 Human
  • Entrez Gene: 10848 Human
  • Entrez Gene: 10848 Human
  • Entrez Gene: 333654 Mouse
  • Omim: 607463 Human
  • SwissProt: Q8WUF5 Human
  • SwissProt: Q8WUF5 Human
  • SwissProt: Q8WUF5 Human

  • SwissProt: Q5I1X5 Mouse
  • SwissProt: Q3TCU2 Mouse
  • Unigene: 466937 Human

  see all

• Alternative names
  IASPP antibodyinhibitor of apoptosis stimulating protein of p53 antibodyInhibitor of ASPP protein antibody

  NFkB interacting protein 1 antibodyNKIP1 antibodyPPP1R13B-like protein antibodyPPP1R13L antibodyProtein iASPP antibodyprotein phosphatase 1 regulatory (inhibitor) subunit 13 like antibodyRAI antibodyRelA-associated inhibitor antibody

  see all