Anti-iASPP antibody [LXO49.3] (ab49805)
- Product nameAnti-iASPP antibody [LXO49.3]See all iASPP primary antibodies ...
- DescriptionMouse monoclonal [LXO49.3] to iASPP
- Tested applicationsWB, ICC, IP more details
- Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to amino acids 459-639 of Human iASPP
- Positive controlTotal cell extract of human osteogenic sarcoma (U-2-OS)
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
- Concentration information loading...
- Purification notesPurified immunoglobulin
- Clonality Monoclonal
- Clone numberLXO49.3
- Research Areas
Our Abpromise guarantee covers the use of ab49805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 92 kDa.|
|ICC||ICC: Use at an assay dependent dilution.|
|IP||IP: Use at an assay dependent dilution.|
- RelevanceASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
- Cellular localizationNuclear
- IASPP antibodyinhibitor of apoptosis stimulating protein of p53 antibodyInhibitor of ASPP protein antibody
- NFkB interacting protein 1 antibodyNKIP1 antibodyPPP1R13B-like protein antibodyPPP1R13L antibodyProtein iASPP antibodyprotein phosphatase 1 regulatory (inhibitor) subunit 13 like antibodyRAI antibodyRelA-associated inhibitor antibody
References for Anti-iASPP antibody [LXO49.3] (ab49805)
ab49805 has not yet been referenced specifically in any publications.