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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab34898 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - iASPP antibody (ab34898)
Anti-iASPP antibody (ab34898) at 1/1500 dilution (at 4°C, o/n) + 35ug of MCF7 whole cell lysate
Secondary
IRDye™800 conjugated anti-Rabbit IgG diluted 1:10000, for 45 min at RT
Predicted band size : 90 kDa
Observed band size : 102 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-iASPP antibody(ab34898)
IHC image of ab34898 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34898, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
iASPP antibody for WB in Human (34898)
iASPP antibody for WB in Rat (34898)
iASPP antibody for WB in African Green Monkey (34898)
iASPP antibody for WB in Hamster (34898)
iASPP antibody for IP in Human (34898)
iASPP antibody for IP in Rat (34898)
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