Our Abpromise guarantee covers the use of ab34898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 102 kDa (predicted molecular weight: 90 kDa).|
|ELISA||1/40000 - 1/160000.|
|IP||Use at 4 µg/mg of lysate.|
Immunoprecipitation analysis of Human HeLa whole cell lysates. The cells were immunoprecipitated untreated with a Protein A matrix diluted at 4 µg/mg lysate incubated for 2 hours at 4°C in RIPA buffer.