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Overview

  • Product nameAnti-iASPP antibodySee all iASPP primary antibodies ...
  • Description
    Rabbit polyclonal to iASPP
  • SpecificityExpect reactivity against both isoform 1 and isoform 2.
  • Tested applicationsIHC-P, WB, ELISA, IP more details
  • Species reactivity
    Reacts with: Rat, Human, Monkey
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide: R EGESVTVLRR DGPEETD, corresponding to amino acids 780-797 of Human iASPP (Isoform 1).

  • Positive controlMCF7 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab34898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB WB: 1/1000 - 1/5000. Detects a band of approximately 102 kDa (predicted molecular weight: 90 kDa).
ELISA ELISA: 1/40000 - 1/160000.
IP IP: Use at 4 µg/mg of lysate.

Target

  • RelevanceASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
      IASPP antibodyinhibitor of apoptosis stimulating protein of p53 antibodyInhibitor of ASPP protein antibody
      NFkB interacting protein 1 antibodyNKIP1 antibodyPPP1R13B-like protein antibodyPPP1R13L antibodyProtein iASPP antibodyprotein phosphatase 1 regulatory (inhibitor) subunit 13 like antibodyRAI antibodyRelA-associated inhibitor antibody
    see all

Anti-iASPP antibody images

  • Anti-iASPP antibody (ab34898) at 1/1500 dilution (at 4°C, o/n) + 35ug of MCF7 whole cell lysate

    Secondary
    IRDye™800 conjugated anti-Rabbit IgG diluted 1:10000, for 45 min at RT

    Predicted band size : 90 kDa
    Observed band size : 102 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab34898 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34898, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-iASPP antibody (ab34898)

This product has been referenced in:
  • Wang L  et al. iASPPsv antagonizes apoptosis induced by chemotherapeutic agents in MCF-7 cells and mouse thymocytes. Biochem Biophys Res Commun 424:414-20 (2012). WB . Read more (PubMed: 22766503) »

See 1 Publication for this product

Product Wall

Displaying 1 - 8 of 8 results for Abreviews and Q&A

Unfortunately we cannot provide the iASPP peptide used as an immunizing peptide. I have found a link to the iASPP EST profile on Unigene that may help you select a negative control.

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglis...

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Thank you for your inquiry.



Please let me know how many vials you would like to order and I will be happy to forward this to the marketing department. They will be happy to provide a quotation for more than 10 vials.

I am sor...

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Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (PC12)
Total protein in input 500 µg
Specification PC12
Immuno-precipitation step Protein A
Username

Verified customer

Submitted Oct 30 2007

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa S3)
Total protein in input 500 µg
Specification HeLa S3
Immuno-precipitation step Protein A
Username

Verified customer

Submitted Oct 30 2007

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Verified customer

Submitted Sep 20 2007

Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 30 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Verified customer

Submitted Sep 20 2007

Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 30 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Verified customer

Submitted Jun 22 2007

Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Loading amount 20 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Verified customer

Submitted Jun 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"