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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-iASPP antibody
See all iASPP products (3) ...
Description
Rabbit polyclonal to iASPP
Specificity
Expect reactivity against both isoform 1 and isoform 2.
Tested applications
IHC-P, WB, ELISA, IPmore details
Cross reactivity
Reacts with
Rat, Human, Monkey
Predicted to work with
Mouse, Cow
Immunogen
Synthetic peptide: R EGESVTVLRR DGPEETD, corresponding to amino acids 780-797 of Human iASPP (Isoform 1).
R EGESVTVLRR DGPEETD
Positive control
MCF7 whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB: 1/1000 - 1/5000.Detects a band of approximately 102 kDa (predicted molecular weight: 90 kDa).
ELISA: 1/40000 - 1/160000.
IP: Use at 4 µg/mg of lysate.
Target
Relevance
ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
Cellular localization
Nuclear
Alternative names
- IASPP antibody
- inhibitor of apoptosis stimulating protein of p53 antibody
- Inhibitor of ASPP protein antibody
see all
Database links
- Entrez Gene: 505818 Cow
- Entrez Gene: 10848 Human
- Entrez Gene: 333654 Mouse
- Omim: 607463 Human
- SwissProt: Q8WUF5 Human
- SwissProt: Q3TCU2 Mouse
- SwissProt: Q5I1X5 Mouse
- Unigene: 466937 Human
Anti-iASPP antibody images:
Western blot - iASPP antibody (ab34898)
Anti-iASPP antibody (ab34898) at 1/1500 dilution (at 4°C, o/n) + 35ug of MCF7 whole cell lysate
Secondary
IRDye™800 conjugated anti-Rabbit IgG diluted 1:10000, for 45 min at RT
Predicted band size : 90 kDa
Observed band size : 102 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-iASPP antibody(ab34898)
IHC image of ab34898 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34898, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-iASPP antibody (ab34898)
ab34898 has not yet been referenced specifically in any publications.
Publishing research using ab34898? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"