iASPP antibody (ab34898)

Product Name 

iASPP antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to iASPP

Immunogen 

Synthetic peptide: R EGESVTVLRR DGPEETD, corresponding to amino acids 780-797 of Human iASPP (Isoform 1).
BLAST 'R EGESVTVLRR DGPEETD' with or

Reacts with 

Hu, Rat, Mk

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Specificity 

Expect reactivity against both isoform 1 and isoform 2.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/40000 - 1/160000.
IP: Use at 4 ug/mg of lysate
WB: 1/1000 - 1/5000. Detects a band of approximately 102 kDa (predicted molecular weight: 90 kDa).
Preincubation with immunizing peptide blocks specific band staining.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 whole cell lysate

Cellular localization 

Nuclear

Research areas 

Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

Relevance 

ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53
proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.190 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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