Anti-iASPP antibody (ab34898)

Overview

• Product nameAnti-iASPP antibodySee all iASPP primary antibodies ...
• Description
  Rabbit polyclonal to iASPP
• SpecificityExpect reactivity against both isoform 1 and isoform 2.
• Tested applicationsIHC-P, WB, ELISA, IP more details
• Species reactivity
  Reacts with: Rat, Human, Monkey
  Predicted to work with: Mouse, Cow
• Immunogen

  Synthetic peptide: R EGESVTVLRR DGPEETD, corresponding to amino acids 780-797 of Human iASPP (Isoform 1).

  Run BLAST with Run BLAST with
• Positive controlMCF7 whole cell lysate

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • p53 pathway

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • p53

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • p53

  • Cell Biology
  • Apoptosis
  • Intracellular
  • p53 Pathway

Applications

Target

• RelevanceASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 505818 Cow
  • Entrez Gene: 10848 Human
  • Entrez Gene: 10848 Human
  • Entrez Gene: 10848 Human
  • Entrez Gene: 333654 Mouse
  • Omim: 607463 Human
  • SwissProt: Q8WUF5 Human
  • SwissProt: Q8WUF5 Human

  • SwissProt: Q8WUF5 Human
  • SwissProt: Q5I1X5 Mouse
  • SwissProt: Q3TCU2 Mouse
  • Unigene: 466937 Human

  see all

• Alternative names
  IASPP antibodyinhibitor of apoptosis stimulating protein of p53 antibodyInhibitor of ASPP protein antibody

  NFkB interacting protein 1 antibodyNKIP1 antibodyPPP1R13B-like protein antibodyPPP1R13L antibodyProtein iASPP antibodyprotein phosphatase 1 regulatory (inhibitor) subunit 13 like antibodyRAI antibodyRelA-associated inhibitor antibody

  see all

Anti-iASPP antibody images

• 

  Western blot - iASPP antibody (ab34898)
  Anti-iASPP antibody (ab34898) at 1/1500 dilution (at 4°C, o/n) + 35ug of MCF7 whole cell lysate
  
  Secondary
  IRDye™800 conjugated anti-Rabbit IgG diluted 1:10000, for 45 min at RT
  
  Predicted band size : 90 kDa
  Observed band size : 102 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-iASPP antibody(ab34898)
  IHC image of ab34898 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34898, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.