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Products:Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
Overview
Product name
Anti-iNOS antibody [NOS-IN]
See all iNOS products (11) ...
Description
Mouse monoclonal [NOS-IN] to iNOS
Specificity
This antibody reacts with iNOS
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide: FSYGAKKGSALEEPKATRL conjugated to KLH, corresponding to amino acids 1126-1144 of Mouse iNOS
FSYGAKKGSA LEEPKATRL
Positive control
Whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-gamma
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
NOS-IN
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
WB
Application notes
WB: 1/1000. Predicted molecular weight: 131 kDa.
Array: Use at an assay dependent concentration.
ICC/IF: 1/1:100 - 1/1:500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Target information above from: UniProt accessionP35228
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- inducible antibody
- HEP NOS antibody
- HEP-NOS antibody
see all
Database links
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 2893 Mouse
- Unigene: 10400 Rat
References for Anti-iNOS antibody [NOS-IN] (ab49999)
This product has been referenced in:
- Ripoll CBet al. Mesenchymal Lineage Stem Cells Have Pronounced Anti-Inflammatory Effects in the Twitcher Mouse Model of Krabbe's Disease. Stem Cells 29:67-77 (2011). WB; Mouse.Read more (PubMed: 21280158) »
- Sabri Met al. Uncoupling of endothelial nitric oxide synthase after experimental subarachnoid hemorrhage. J Cereb Blood Flow Metab 31:190-9 (2011). WB; Mouse.Read more (PubMed: 20517322) »
See all 6 publications for this product
Publishing research using ab49999? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"