iNOS antibody (ab15323)

Product Name 

iNOS antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to iNOS

Immunogen 

Synthetic peptide: CFSYGAKKGSALEEPKATRL, corresponding to C terminal amino acids 1125-1144 of Mouse iNOS.
BLAST 'CFSYGAKKGSALEEPKATRL' with or

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at an assay dependent dilution (PMID 18523253).
IHC-P: 1/100. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 131 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Lung

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS

Relevance 

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C.

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iNOS antibody images:

References for iNOS antibody (ab15323)

  This product has been used in: (two most recent references)

Eubank TD et al. Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages. Cancer Res 69:2133-40 (2009). IHC-P; Human. PubMed: 19223554

Dugast AS et al. Myeloid-derived suppressor cells accumulate in kidney allograft tolerance and specifically suppress effector T cell expansion. J Immunol 180:7898-906 (2008). WB, IHC-Fr; Rat. PubMed: 18523253

See all 7 publication references for this product.


If you publish research using ab15323 please let us know so that we can cite the reference on this datasheet.

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