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Products:Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
Overview
Product name
Anti-iNOS antibody
See all iNOS products (11) ...
Description
Rabbit polyclonal to iNOS
Specificity
This antibody detects iNOS. It does not detect other NOS isoforms. By Western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. By Western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples.
Tested applications
ICC/IF, IHC-P, IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Dog, Human, Xenopus laevis
Immunogen
Synthetic peptide: DLKEEKDINNNVKKT, corresponding to amino acids 17-31 of Mouse iNOS.
DLKEEKDINN NVKKT
Positive control
WB: recombinant human or mouse iNOS, lysate of cytokine stimulated A549 cells, lysate of cytokine stimulated RAW 264.7 cells, lysate of cytokine stimulated rat fibroblasts, lipopolysaccharide-treated mouse spleen
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/50ICC/IF: 1/50
IHC-P: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20708681)
WB: 1/200Detects a band of approximately 135 kDa.(Note: Regarding using this antibody in WB on mouse lung tissue: "However, iNOS protein was below the limit of detection following any level of hypoxic exposure (positive control was lipopolysaccharide-treated mouse spleen) and using several different antibodies" (from PubMed ID 11686901))
Target
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Target information above from: UniProt accessionP35228
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- inducible antibody
- HEP NOS antibody
- HEP-NOS antibody
see all
Database links
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 709191 Human
see all
Anti-iNOS antibody images:
- iNOS antibody (ab3523)
IHC with a indirect immunoalkaline phosphatase (APAAP) technique using ab3523. Shows iNOS expression within vessels walls in the Locus Coeruleus after death from septic shock. Picture kindly given to us by Prof. Djillali Annane.
Western blot - iNOS antibody (ab3523)
All lanes : Anti-iNOS antibody (ab3523) at 1/3000 dilution
Lane 1 : Whole tissue lysate prepared from mouse lungs
Lane 2 : Whole tissue lysate prepared from mouse lungs
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/10000 dilution
developed using the ECL technique
Observed band size : 131 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab3523)
ab3523 staining iNOS in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde, blocked with 5% BSA for 30 minutes and then incubated with ab3523 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
Image courtesy of an anonymous Abreview.
References for Anti-iNOS antibody (ab3523)
This product has been referenced in:
- Burggraaf Set al. Increased Inducible Nitric Oxide Synthase Expression in Organs Is Associated with a Higher Severity of H5N1 Influenza Virus Infection. PLoS One 6:e14561 (2011). IHC-P; Chicken, Duck.Read more (PubMed: 21283521) »
- Tsuhako MHet al. Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation. Free Radic Biol Med 48:704-12 (2010). IHC-P; Mouse.Read more (PubMed: 20035861) »
See all 12 publications for this product
Publishing research using ab3523? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"