iNOS antibody (ab3523)

Product Name 

iNOS antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to iNOS

Immunogen 

Synthetic peptide: DLKEEKDINNNVKKT, corresponding to amino acids 17-31 of Mouse iNOS.
BLAST 'DLKEEKDINNNVKKT' with or

Reacts with 
(species key)

Hu, Ms, Rat, Dog, Xl

Specificity 

This antibody detects iNOS. It does not detect other NOS isoforms. By Western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS and human iNOS from cytokine stimulated A549 cells. By Western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells and cytokine stimulated rat fibroblast iNOS. However, the signals are not as strong as those seen with the human samples.

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/50.
IHC-P: Use at an assay dependent dilution.
WB: 1/200. Detects a band of approximately 135 kDa.
Note: Regarding using this antibody in WB on mouse lung tissue: "However, iNOS protein was below the limit of detection following any level of hypoxic exposure (positive control was lipopolysaccharide-treated mouse spleen) and using several different antibodies" (from PubMed ID 11686901)


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: recombinant human or mouse iNOS, lysate of cytokine stimulated A549 cells, lysate of cytokine stimulated RAW 264.7 cells, lysate of cytokine stimulated rat fibroblasts

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Nitric Oxide >> NOS

Relevance 

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS, 1 mg/ml BSA, 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

4.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for iNOS antibody images, references, products related to ab3523 and other tools.

iNOS antibody images:

References for iNOS antibody (ab3523)

  This product has been used in: (two most recent references)

Tsuhako MH et al. Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation. Free Radic Biol Med 48:704-12 (2010). IHC-P; Mouse. PubMed: 20035861

Innamorato NG et al. The Transcription Factor Nrf2 Is a Therapeutic Target against Brain Inflammation. J Immunol 181:680-9 (2008). WB; Mouse. PubMed: 18566435

See all 11 publication references for this product.


If you publish research using ab3523 please let us know so that we can cite the reference on this datasheet.

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