Overview

  • Product nameAnti-IARS2 antibody
    See all IARS2 primary antibodies
  • Description
    Rabbit polyclonal to IARS2
  • Specificityab66012 detects endogenous levels of total IARS2 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human IARS2.

  • Positive control
    • IHC-P: Human breast carcinoma tissue ICC/IF: HepG2 cells WB: Extracts from RAW264.7 cells and COS7 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab66012 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).

Target

  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010002H18Rik antibody
    • C79125 antibody
    • EC 6.1.1.5 antibody
    • FLJ10326 antibody
    • Iars2 antibody
    • IleRS antibody
    • isoleucine tRNA ligase 2, mitochondrial antibody
    • isoleucine tRNA ligase, mitochondrial antibody
    • isoleucine tRNA synthetase 2, mitochondrial antibody
    • Isoleucine--tRNA ligase antibody
    • isoleucine-tRNA synthetase 2 antibody
    • isoleucyl tRNA synthetase 2, mitochondrial antibody
    • Isoleucyl-tRNA synthetase antibody
    • MGC63429 antibody
    • mitochondrial antibody
    • mitochondrial isoleucine tRNA synthetase antibody
    • rCG_20084 antibody
    • RGD1311857 antibody
    • SYIM_HUMAN antibody
    see all

Anti-IARS2 antibody images

  • ab66012 at 1/500 dilution staining IARS2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab66012, at a 1/50 dilution, staining IARS2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
  • All lanes : Anti-IARS2 antibody (ab66012) at 1/500 dilution

    Lane 1 : RAW264.7 cell extracts
    Lane 2 : COS7 cell extracts
    Lane 3 : COS7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 114 kDa
    Observed band size : 114 kDa

References for Anti-IARS2 antibody (ab66012)

ab66012 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"