Overview

  • Product nameAnti-iASPP antibody [LXO49.3]
    See all iASPP primary antibodies
  • Description
    Mouse monoclonal [LXO49.3] to iASPP
  • Tested applicationsWB, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 459-639 of Human iASPP

  • Positive control
    • Total cell extract of human osteogenic sarcoma (U-2-OS)

Properties

Applications

Our Abpromise guarantee covers the use of ab49805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 92 kDa.
ICC Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • RelevanceASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • IASPP antibody
    • inhibitor of apoptosis stimulating protein of p53 antibody
    • Inhibitor of ASPP protein antibody
    • NFkB interacting protein 1 antibody
    • NKIP1 antibody
    • PPP1R13B-like protein antibody
    • PPP1R13L antibody
    • Protein iASPP antibody
    • protein phosphatase 1 regulatory (inhibitor) subunit 13 like antibody
    • RAI antibody
    • RelA-associated inhibitor antibody
    see all

References for Anti-iASPP antibody [LXO49.3] (ab49805)

ab49805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"