Anti-Iba1 antibody [1022-5] (FITC) (ab15691)

Overview

  • Product nameAnti-Iba1 antibody [1022-5] (FITC)
    See all Iba1 primary antibodies
  • Description
    Mouse monoclonal [1022-5] to Iba1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab15691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.
  • Application notesIs unsuitable for IHC.
  • Target

    • FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
    • Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
    • Sequence similaritiesContains 2 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIF 1 antibody
      • AIF 1 antibody
      • AIF-1 antibody
      • AIF1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein G1 antibody
      see all

    Anti-Iba1 antibody [1022-5] (FITC) images

    • Overlay histogram showing THP1 cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10µl/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10µl/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References for Anti-Iba1 antibody [1022-5] (FITC) (ab15691)

    This product has been referenced in:
    • Schluesener HJ  et al. Effects of autoantigen and dexamethasone treatment on expression of endothelial-monocyte activating polypeptide II and allograft-inflammatory factor-1 by activated macrophages and microglial cells in lesions of experimental autoimmune encephalomyelitis, neuritis and uveitis. Acta Neuropathol (Berl) 97:119-26 (1999). Read more (PubMed: 9928822) »
    • Schluesener HJ  et al. Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells. Glia 24:244-51 (1998). Read more (PubMed: 9728770) »

    See all 2 Publications for this product

    Product Wall

    I have spoken to our custom order specialist and they have told me that if you would like 1mg of ab15690 conjugated to PE, then the cost would be over $3000.00. If you would like to go ahead and place a custom order for ab15690, please let me know and ...

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    Thank you for your reply.

    I have forward your request about the quote for 1mg of ab15690 conjugated to PE to our in house specialist and I will let you know as soon as I hear back from them.

    As to other conjugates that would work fo...

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    Application Flow Cytometry
    Sample Mouse Cell (Brain)
    Specification Brain
    Fixation Paraformaldehyde
    Permeabilization Yes - BD Perm/Wash
    Gating Strategy Microglia
    Username

    Abcam user community

    Verified customer

    Submitted Feb 01 2012

    Application Flow Cytometry
    Sample Mouse Cell (spinal cord neuron)
    Specification spinal cord neuron
    Preparation Cell harvesting/tissue preparation method: primary cell culture
    Sample buffer: 0.5% BSA + 2 mM EDTA/PBS without divalent cations
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy macrophages
    Username

    Ms. Minjung Kwon

    Verified customer

    Submitted Jan 21 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"