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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Rat Iba1.
Our Abpromise guarantee covers the use of ab107159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 25068145|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Abcam recommends blocking in milk (3-5%) for cleaner blots with reduced background, in comparison to BSA.
Lanes 1-14: Blocked in 3% milk for 1 hour (RT).
Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab107159 (anti-Iba1 antibody; 2 ug/mL) for 18 hours at 4°C. Antibody binding was detected using a Dako HRP-labelled rabbit anti-goat IgG at 1:5,000 dilution for 1 hour at room temperature and visualised using ECL development solution ab133406.
ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.