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C-NKQFLDDPKYSSDED, corresponding to internal sequence amino acids 28-42 of Human Iba1 (NP_001614.3)
Our Abpromise guarantee covers the use of ab48004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
We recommend blocking in 3-5% milk, not BSA. Blocking in BSA gives high background in WB.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab48004 (2µg/ml) staining of paraffin embedded Mouse Brain shows staining of astrocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Primary incubation was 1 hour
ab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature. Incubation time with the primary antibody was 16 minutes. A biotinylated rabbit anti-goat was used as the secondary
ab48004 has not yet been referenced specifically in any publications.