Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Iba1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P55008
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab178680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
Abcam recommends blocking in 3% milk for this product. Blocking in BSA gives high background.
IHC image of Iba1 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab178680, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Iba1 with ab178680 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Iba1 with ab178680 at 1/250 dilution.
Immunofluorescent analysis of K562 cells labeling Iba1 with ab178680 (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized K562 cells labeling Iba1 with ab178680 at 1/100 dilution (red) or a rabbit IgG (negative) (green).