Recombinant Anti-Iba1 antibody [EPR6136(2)] (ab178680)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6136(2)] to Iba1
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Iba1 antibody [EPR6136(2)]
See all Iba1 primary antibodies -
Description
Rabbit monoclonal [EPR6136(2)] to Iba1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: THP-1 cell lysate. Flow Cyt (Intra): K-562 cells. ICC/IF: Jurkat cells. IHC-P: Human kidney, Human tonsil, human glioma and Human cerebrum tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6136(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/100.
|
|
IHC-P |
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
|
WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
|
Notes |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
IHC-P
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
Target
-
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
Tissue specificity
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
Sequence similarities
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
-
Database links
- Entrez Gene: 199 Human
- Omim: 601833 Human
- SwissProt: P55008 Human
- Unigene: 76364 Human
-
Alternative names
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
Images
-
Immunohistochemical analysis of Paraffin-embedded sections Human tonsil tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (BondTM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BondTM Polymer Refine Detection).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human tonsil.
The section was incubated with ab178680 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
Immunohistochemical analysis of Paraffin-embedded sections Human glioma tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (BondTM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BondTM Polymer Refine Detection).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human glioma.
The section was incubated with ab178680 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
Immunohistochemical analysis of Paraffin-embedded sections Human cerebrum tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (BondTM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BondTM Polymer Refine Detection).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human cerebrum.
The section was incubated with ab178680 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
-
Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Iba1 with purified ab178680 at 1:100 dilution (9.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Anti-Iba1 antibody [EPR6136(2)] (ab178680) at 1/1000 dilution (Purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
-
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Iba1 with purified ab178680 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (25)
ab178680 has been referenced in 25 publications.
- Zhao T et al. Folic Acid Attenuates Glial Activation in Neonatal Mice and Improves Adult Mood Disorders Through Epigenetic Regulation. Front Pharmacol 13:818423 (2022). PubMed: 35197855
- Zhao P et al. LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions. Mol Neurodegener 17:44 (2022). PubMed: 35717259
- Dhillon SK et al. Adverse neural effects of delayed, intermittent treatment with rEPO after asphyxia in preterm fetal sheep. J Physiol 599:3593-3609 (2021). PubMed: 34032286
- Wassink G et al. Recombinant erythropoietin does not augment hypothermic white matter protection after global cerebral ischaemia in near-term fetal sheep. Brain Commun 3:fcab172 (2021). PubMed: 34409290
- Rey F et al. Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinson's Disease. Antioxidants (Basel) 10:N/A (2021). PubMed: 33467745