Selective blocker of the high-conductance, Ca2+-activated K+ channel
Biological descriptionSelective blocker of the high-conductance, Ca2+-activated K+ channel. Originally isolated from the scorpion Mesobuthus tamulus. Specifically blocks KCa1.1 (Slo) channels (Ki approx. 1 nM).
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 0.7 mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
HEK293 cells plated for 48 hours and then transfected with rat Slo27 for 24 hours. Currents from whole-cell recordings, with 1 µM Ca2+ in the electrode solution. Cells voltage-clamped at -80 mV, with currents activated by 500 ms depolarization to +20 mV. Concentration-inhibition relationship produces an IC50 of 412 pM.
(Number of experimental determinations shown in brackets).
References for Iberiotoxin (ab120379)
This product has been referenced in:
Meera P et al. A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin. Proc Natl Acad Sci U S A97:5562-7 (2000).
Read more (PubMed: 10792058) »
Koschak A et al. [125I]Iberiotoxin-D19Y/Y36F, the first selective, high specific activity radioligand for high-conductance calcium-activated potassium channels. Biochemistry36:1943-52 (1997).
Read more (PubMed: 9048582) »